Deep sequecing of Avian coronavirus Beaudette strain at 1st passage... - SRA

SRX13829058: Deep sequecing of Avian coronavirus Beaudette strain at 1st passage in VERO cells
1 ILLUMINA (NextSeq 500) run: 8M spots, 2G bases, 825.3Mb downloads

Design: ds-cDNAs were prepared using random primers, SuperScript III Reverse Transcriptase (Invitrogen) and 3-5 exo Klenow DNA Polymerase (Ambion) as per manufacturer and libraries were obtained using Nextera Sample Preparation (Illumina), purified with magnetic beads using AMPure XP (Beckman Coulter Life Sciences) and quantified with qPCR using KAPA Fast Universal (Sigma-Aldrich). Reads were then obtained in a NextSeq500 MID Output 300 System (Illumina) (2 x 150pb).

Submitted by: School of Veterinary Medicine

Study: Avian coronavirus strain:Beaudette Genome sequencing and assembly

PRJNA736341 • SRP323478 • All experiments • All runs

show Abstracthide Abstract

A study on Avian coronaviurus diversity at the mutant spectrum level under antibody neutralizaton and spillover.

Sample: Pathogen: clinical or host-associated sample from Avian coronavirus

SAMN19643026 • SRS9171275 • All experiments • All runs

Organism: Avian coronavirus

Library:

Name: Beaudette strain VERO 1P

Instrument: NextSeq 500

Strategy: WGS